Plurk paste

Anyway
5Min Drop I don't think really mixed much at all becauseeeee there sure is just a blob of indigo in the middle of pale yellow.

10Min Drop is predominantely a muddy brown with a slight edge of greenyblack

15Min Drop is darker at the brown point, but has a rim of light blue around the mess, how lovely...

20Min Drop is the same sort of brown that 10 min was, which is about the usual sign that I have done something not quite right in this lab.

25Min Drop is also doing the orangy brown deal, which. Yeah. Not a great sign! Notably, 15min Drop at this point is almost ENTIRELY blue-black. I can't tell if that's because it's touched marker ink (I doubt it though because 10Min is right over it, and hasn't done anything), or if maybe my amylase and starch were somehow not as mixed up as expected?

30Min Drop is a very golden colour again...Looking online supposedly the point is that eventually the starch is digested by amylase and therefore the iodine wouldn't react but ??? That either didn't take long at all or! Something!

Also: I don't have vinegar. In fact, I actually missed vinegar on the first read of this. I used lemon juice, since I suspect the pH is what I need it for.

Halfway through the experiment I cleaned up the other half of my petri dish and started the same experiment but with spit (saliva). Just. In. Case.

Unfortunately, saliva is really hard to work with annnnd therefore there is more concentration of that on ten minutes and 30 minutes. So we'll see how that affects it??? Here's hoping this round with lemon juice goes better but anyway.

5Min Saliva Drop- Looks kind of like a fire, we have a lot of golden look...

10Min definitely has too much saliva ahaha...but interestingly, the droplet as a whole took a very nice violet, indigo sheen and even developed a halo of violet. Notably as well, I noticed that the 5min Drop has an edge of black, and black streaks through it too.

15Min, I looked more closely at the droplet- indeed, while the mixture is a muddy brown (more brown than orange this time), blue is *practically fleeing* from the iodine site. Streaks of black can be seen marbling through; the reason it isn't a solid colour is because the starch solution is still at its base water and particulates I think...or maybe it's the consistency of saliva in this case. It is pretty...thick.

20Min in and the 15 minute one has a nice puddle of blue around it, while 10 still has that halo going on. 20 itself has a slight edging and puddle sheen, with the iodine portion itself looking a far more orangey, rusty hue, which I feel is proving my theory on the consistency separating the starch somewhat. I kind of wish my phone had a better camera on it (this is. my only camera) as these look rather beautiful actually.

25Min, and haloing is minimal, though there are definitely streaks still going through. These streaks seem minimal though, and almost brown rather than inky. 5 and 10 by now are practically pale yellow for that matter, while 15 is a weird green surrounded by blue. I think the more brown-gold we get, the less starch we have left- this iodine IS brown pretty much...

30Min! We're not doing this again with lemon juice though, the saliva is too hard to work with...This one definitely has an extreme concentration of saliva whoops. The iodine just sort of...pooled through it. That said; I think from this, even with the over econcentrated 10 and 30, we can see how the starch is broken down by the amylase/saliva; the streaks and flecks lessened with time, and in 30 there isn't anything at all. Not even a hint! (If it was purely the oversaturation, 10 wouldn't have had streaks either- but nope, there was some good marbling going on.) The less starch there was, the more mixed everything got too...from a yellowy look to a golden brown.

While doing the saliva run, I started 'part two' with the lemon juice standing in for vinegar. I remade my starch solution (rather, remixed- as the pot wasn't emptied) for this, since I feel the pH strips contaiminated it somehow... (I also tested to make sure it actually reacts and YEP, black on contact with iodine, good sign.)

5Min with Lemon Juice, and it's a weird muddy colour with an edge of green.

10Min, and while we have the muddy colour, I'm prompted to look closer at both water bubbles- the starch flecks and particles are a solid black!! So it is in there...Otherwise though, it's still a sea of brown...

15Min- While the solution is a stronger brown, there are still visible flecks of starch, indicated by black dots. I think the lemon juice is slowing the amylase down, but I can't be sure- after all, at 15 minutes in the other two runs, there was still plenty of black streaking....notably however, there have been no indigo or violet halos in any of these. Maybe the lemon juice's cold temperature is affecting the starch solution itself?

20Min- The number of visible starch particles is disappearing, but the iodine mix is still a heavy brown hue.

25Min- I think the lemon juice is definitely inhibiting the amylase. There has been no change in the particular shade of brown vs particulates- normally by now, the presence of streaks has at least depleted.

30Min- The colour here is a muddy blackish brown, with a few flecks again. All of these drops I've tried to keep away from the numbers...but this one wasn't on it at all, so I know for sure that's starch. The lemon juice definitely inhibited it!

I decided at this point to do One More run- no lemon juice or anything, but rather by mixing some saliva with the amylase solution I had. The idea of course being that it would just be more amylase, and also easier to work with. I only started this after the lemon juice run was finished. Just so I could compare the last results.

The clock is set and my syringe is kinda gross, here we go.

5 Min in and we have a sort of blackening haze with visible specks of black- probably the starch. Notably, I did test this batch of starch mixture BEFORE setting this up. And...the whole droplet went deep navy blue so yeah. Also we have three droplets of starch to one droplet of saliva amylase soooo...

10 Min, and where 5 min was sort of hazy, this droplet starts with a rich brown again with flecks of black where the starch is. I suspect that what's happening is that being exposed to the amylase/saliva mixture is causing the particulates of starch to lose their influence on the water- in the starch mixture, it's just starch and water. Whatever the particles do to the iodine, the water can carry immediately. But here, once it's in some saliva and amylase, the starch seems to group in on itself.

Notably, 10 minutes has a slight halo of violet, next to where many particles seem to have gathered and settled. This seems to support my theory- as the amylase and saliva act on the starch, what's left is gathering elsewhere. The iodine around the amylase holds its brown hue, but goes a slight blue hue around the starch- turning the particles black in the meantime.

15Min, and where 5 seemed to at least blend with the navy hue, and 10 even separated it out in a visible process, 15 seems to come more in shades of brown to gold, with very few visible particles. 10's edge of indigo looks really cool though...after a minute or so, some violet can be seen at the lower end of the droplet. It's the same as 10's sediment effect, but a bit smaller. Supports the fact that Amylase goes through Starch!

20Min AND NOOOOO IT ATE A SPILLED DROP OF STARCH THAT DIDNT HAVE AMYLASE....oh well. In this one we have that dark cloudly look again, but there's already a visible separation of contents- immediately I can see the border edge of indigoblack, while yellow-brown otherwise dominates. This is a different look compared to 5Min, where it was straight up a full cloud of brown-black-blue so. This is definitely following the pattern.

25Min. It's come to my attention that some of these larger particles are MARKER FRAGMENTS so that's embarassing? That said, the colours are still supporting everything. While 20 had some mixing still going on, 25 seems to be nothing but that lovely golden brown outside some flecks of marker. I'm keeping an eye on it, but so far looking back at everything we have a steady loss of purple- and 25 hasn't got a lick of it anymore.

Notably over the course of these 25 minutes btw, the iodine seems to have settled more thoroughly into each solution. 5 is muddy, but visibly a mix of dark hues of indigo. 10 has some separation, but over all a Lot of indigo, while 15 and 20 both have trace amounts of indigo at the edges with obvious starch deposits. I suspect 30 will just have...No starch left to show.

30Min. I was right, but we'll see I'm cleaning everything up and leaving the petri dish to be safe. ...I was right! 30 is still very orange.